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ALEXYS HPLC/ECD
Applications
ECD, flow cell, options
HPLC and sensitivity
PC and software
ROXY for EC/MS
Applications
Acetylcholine
GABA, Glu, bad peak shape, loss of signal
Netilmicin
Phenols
Thiols, disulfides, bad peak shape, loss of signal on BDD diamond electrodes
VMA
Working with a silver working electrode
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ALEXYS HPLC/ECD
Autosampler
AS 100 autosampler down, several error messages
AS 100 autosampler takes up more sample than expected
AS 100 error: spindle does not rotate
Excessive peak broadening with partial loop fill
No peaks, no sample is introduced
Poor reproducibility - autosampler issues
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Pump
How to change the IP address of a pump P6.1L?
I hear a strange squeaky noise
Pump error: leak detected
Pump LC110S does not connect
How to switch off an HPLC system?
OQ or PQ is 'failed'
Pressure increase in HPLC
Should I switch off my detector when not in use?
ROXY for EC/MS
I do not get much reaction products
Why is my diamond/carbon electrode broken?
ECD, flow cell, options
Cells, checks and maintenance
Can I connect an Antec flow cell to another brand ECD?
Can I use an ISAAC reference electrode?
Electrochemical electrode cleaning
Flow cell contact test
Gas bubbles in flow cell
How can I check the performance of my flow cell?
How often must I clean my flow cell electrodes?
How strongly must I tighten the flow cell bolts?
ISAAC shows light colored spots
Stop flow test
What is the best position of my flow cell?
Which reference electrode do you recommend?
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ECD checks and issues
Cannot connect to Elite, IP address is different
DECADE Elite does not connect to USB port
DECADE II displays an error code
DECADE II sensor board dip switches
DECADE II shows 'ERR 20' or 'Initializing'
Detector out of range or PAD overload
Detector oven is not heating
Detector temperature out of spec
Dummy cell test
Dummy cell test failed
Dummycell test failed, internal dummycell test passed
How to apply grounding in the flow path
How to change the IP address of a DECADE Elite?
Input contact test - IO test
Should I switch off my detector at night?
Stop flow test
Time file event not executed
Will the 10V output of my detector damage my 1 V data system?
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Conditions and methods
Can I use my detector in strongly alkaline or acid media?
Can I use Teflon for low pressure tubing?
Is it sufficient to thermostat my flow cell, not the column?
What precautions should I take when measuring in the reductive mode?
Why do I need a Faraday shielded oven?
Change of product notice
External valve not responding
What does the 5 year warranty on the flow cell mean?
HPLC and sensitivity
Noise, drift and cell current
After injecting I see spikes in the chromatogram
Baseline shows pressure pulsations
Cell current shows large continuous drift
Continuous overload of cell current
High background current
Negative peaks in my dialysates interfere with my analysis
Too much noise on my baseline
Why do I see steps in my baseline?
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Peak shape, height and sensitivity
After installing a (new) column the sensitivity is lost
Decrease in detector response
Excessive peak broadening with partial loop fill
How to improve my detection limit?
My peaks are (much) wider than to be expected
No signal
Small peaks compared to literature
What detection limits are feasible in HPLC/ECD?
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Repeatability and linearity
ALEXYS system with poor reproducibility
My bio-inert HPLC system for carbohydrates show a rapid decrease in peak heights
Poor reproducibility - autosampler issues
Small injection volumes, non-linear results
Analysis of main and by-products in one chromatogram
How do I know if a substance is EC detectable?
How much volume can I inject on my HPLC system?
How to prepare and install a new column?
What kind of mobile phase filters must I use in LC EC?
PC and software
Clarity
Migration of Clarity to a new PC
Sample queue stops unexpectedly using Clarity
Start log file in Clarity
Sum of selected files and already open files exceeds specified overlay limit.
What Clarity files are needed for support questions?
Antec ECD driver problem with OpenLab
Antec ECD driver problems with Chromeleon
Can I use an USB to RS232 convertor ?
Connecting a start trigger for Agilent AD converter 35900E
Data acquisition stops, LAN network connection problem
Dialogue Elite shows error with red cross in graph
PC requirements
What cable do I need to connect my device to a PC?
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