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Applications » Diagnostics » Iodide in urine

Iodide in urine


  • HPLC with RP C18 and ion pair
  • Extremely selective Ag electrode
  • Quick and easy sample prep
  • Robust & reproducible

A method for the determination of iodide is developed using electrochemical detection (ECD) with a silver working electrode (WE). The method consists of reversed phase chromatography using an ion pair and phosphate/citrate buffer at pH 6.5, followed by amperometric detection at 0.15 V. The silver electrode is extremely selective towards iodide, which is detected at a potential close to zero. As a result the sample prep is easy, only a dilution and filtration step of the urine samples. The chromatogram shows a triplicate analysis of a urine sample containing about 2 µmol/L iodide.