Choline and Acetylcholine
HPLC analysis of Choline and Acetycholine
- Total analysis time < 10 min
- Small sample volume: 5µL
- Detection limit 1.5 fmol on column (0.3 nmol/L)
The ALEXYS Neurotransmitter Analyzer can be used for fast and sensitive detection of basal acetylcholine (ACh) levels in microdialysis samples. Analysis of ACh by HPLC-ECD is based on an ion-pairing separation, followed by post-column enzymatic conversion to hydrogen peroxide with acetylcholinesterase (AChE) and choline oxidase (ChO). Both enzymes are covalently bound to a stationary phase in an immobilized enzyme reactor (IMER). After conversion, the hydrogen peroxide is detected using the DECADE Elite electrochemical detector with Sencell on a Pt electrode. The method is optimized for analysis of small sample volumes at low nanomolar detection limit.
Neurotransmitter applications
| Title | Version | Size | Download |
|---|---|---|---|
| 211_004 - Glutathione, other Thiols, and Disulfides | 07 | 929.75 KB | Download |
| 221_253 - Fast analysis of Dopamine and Serotonin for high time resolution | 01 | 4.33 MB | Download |
| 211_005 - Aminothiols, Glutathione and disulfides | 02 | 545.38 KB | Download |
| 213_020 - GABA and glutamate | 12 | 1.74 MB | Download |
| 213_021 - Histamine | 03 | 408.04 KB | Download |
| 213_022 - Large Neutral Amino Acids | 01 | 507.15 KB | Download |
| 213_023 - Acetylcholine and Choline | 14 | 0.96 MB | Download |
| 213_028 - Monoamines and Metabolites | 09 | 1.17 MB | Download |
| 213_029 - OMD of neurotransmitters | 04 | 1.29 MB | Download |
| 213_030 - 3–Hydroxykynurenine | 02 | 444.02 KB | Download |
| 213_032 - Tyrosine, Tyramine and Octopamine | 01 | 353.84 KB | Download |