Select Page
Welcome to our Knowledge Base

Netilmicin

You are here:
< All Topics

Applies to

Netilmicin analysis according to EP 9.2, Au electrode, application note 217_031, aminoglycoside antibiotics

Summary

Shift in retention times, high noise and bad peak shape in Netilmicin Sulphate analysis according to EP 9.2 method

Cause and remedy

  • Retention time. The method for analysis of netilmicin sulphate according to the EP9.2 method is based on ion-pairing HPLC separation on a C18 column. A significant shift in retention time can be experienced when working under the specified conditions without regular regeneration of the column or when the column was not stored properly. Flush/regenerate the column on a regular basis according to the instructions in the column user manual when retention times and separation are deteriorating too much. Also be aware that ion-pairing separation takes more time to stabilize..
  • Noise: a specific source of noise for this application is the combination of a saltbridge reference electrode in the flow cell and sodium hydroxide solution needed for detection in PAD-mode. The cotton frit in this reference electrode can get contaminated in a short period of time, resulting in increased baseline noise levels. A regular replacement of the cotton frit is advised. An alternative reference electrode (but not conform the EP method) is our maintenance-free HyREF. See out other kb paper about high noise levels for more general possible causes
  • Bad peak shape: overloading the system with high concentrations of netilmicin can resulted in distorted peak shapes. The use of highest AST setting nr 3 in case of the SenCell or 130 um spacer in case of the FlexCell result in best peak shapes.
  • Bad peak shape: a contamination on the auxiliary electrode or gold electrode can affect the peak shape. Polishing and cleaning these electrodes is advised.

 

Was this article helpful?
0 out Of 5 Stars
5 Stars 0%
4 Stars 0%
3 Stars 0%
2 Stars 0%
1 Stars 0%
How can we improve this article?
Please submit the reason for your vote so that we can improve the article.
Previous GABA, Glu, bad peak shape, loss of signal
Next Phenols
Table of Contents