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No peaks, no sample is introduced

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Applies to

HPLC with autosampler


No, or too small signal/front peaks visible in chromatogram.


Blockage of AS flow path or leakage in the injection valve.

Diagnosis and Remedy

Follow the trouble-shoot instructions in the flow diagram below. See also: ‘no signal‘ for detector related issues.  


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Previous Excessive peak broadening with partial loop fill
Next Poor reproducibility – autosampler issues
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