Select Page
Welcome to our Knowledge Base

After installing a (new) column the sensitivity is lost

You are here:
< All Topics

Applies to

Electrochemical detector

Summary

Immediately after installation of a (new) column a relatively high cell current is observed, the baseline drifts continuously and is noisy.

Cause

Installation of a new or used column

Diagnosis

The problems started with exchanging the column.

Remedy

A new column usually contains traces of contaminants from the production process. In UV methods this is not a problem but in ECD it contaminates the electrode and results in deterioration of performance. Prior to using a new column check the manufacturer’s instructions for compatible washing solutions. For reversed phase columns washing out with methanol/water (80/20) or similar for 24 hrs at a low flow rate is recommended. Disconnect the cell during this process. If a column is installed that was previously used for non-EC applications the same procedure is recommended since LC-EC conditions are usually quite different.

Read more in this KB paper…

Was this article helpful?
0 out Of 5 Stars
5 Stars 0%
4 Stars 0%
3 Stars 0%
2 Stars 0%
1 Stars 0%
How can we improve this article?
Please submit the reason for your vote so that we can improve the article.
Next Decrease in detector response
Table of Contents