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Sum of selected files and already open files exceeds specified overlay limit.
When opening a number of chromatograms (overlay mode) a message is shown, numbers per example: Sum of selected files (44) and already open files (1) exceeds specified overlay limit (20). Only the first 20 files are opened.
From the instrument window, go to the View/Options menu. In the ‘general’ tab set the max file number as desired.