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Carbohydrates

ALEXYS Carbohydrate Analyzer

The ALEXYS Carbohydrate Analyzer is based on High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). Dedicated flow cells are available – FlexCell and SenCell – with high pH resistance, and maintenance free HyREF reference electrode. Together with a Helium degassing kit for carbon dioxide free mobile phase, this assures most stable and sensitive analysis conditions. Applications are available for analysis of carbohydrates in food, beverages, plants, lactose free products, glycoproteins and others. Detection limits as low as 10 nmol/L can be obtained. See also our application notes at the bottom of this page.

Electrochemical detection of carbohydrates is done in the ‘pulse mode’. This is necessary, because EC detection of carbohydrates results in oxidation products that stick to the electrode surface. In PAD a cleaning step is applied which ensures stable and reproducible detection conditions.

For more details,

ALEXYS Carbohydrate Analyzer
System reproducibility – overlay of consecutive injections (n=10).

Click to read brochure.

ET 210 Eluent tray

High Performance Anion Exchange Chromatography (HPAEC) is used for HPLC analysis of carbohydrates. It is only at very high pH that carbohydrates are anions and can be separated in ion chromatography. Therefore, only a few HPAEC columns can be used as they have to be used at pH 12 or higher.

At such high pH, carbon dioxide from the surrounding air dissolves in the mobile phase and forms carbonate ions. The slowly rising concentration of carbonate enters the column, it occupies functional groups of the stationary phase and changes the retention times of carbohydrates. It is therefore most important to have proper carbon dioxide removal from the mobile phase to ensure reproducible separation conditions.

The ALEXYS eluent tray has the infrastructure for keeping the solvent bottles under a blanket of an inert gas. Gas tight caps, tubing, fittings and a manometer to adjust the gas flow and to keep the gas consumption to an absolute minimum.

SweetSep – The New Benchmark for Carbohydrate Analysis

SweetSep is a new line of High-Performance Anion Exchange (HPAE) columns for fast and superior separation of mono-, oligo- and polysaccharides using PAD or MS detection. SweetSep is optimized for the analysis of a wide variety of carbohydrates in food and structural analysis of protein glycosylation (glycoproteins).

For more details, visit the SweetSep product page.

Analysis of 15 sugar standards on a SweetSep AEX200 column using HPAEC-PAD.

Food and Beverage

 

Rev
Name
Size
Download
220_002_11
Carbohydrates in food products
1.77 MB
220_003_09
Mono- and disaccharides
1.37 MB
220_006_08
Carbohydrates in instant coffee
1.21 MB
220_016_06
Carbohydrates in food according to AOAC
2.10 MB
220_019_07
Sugars in meat and fish
2.04 MB
220_025_05
Carbohydrates in honey
1.49 MB
220_028_02
Oligo- Polysaccharides in honey
0.96 MB
220_029_02
Oligo- Polysaccharides in honey and agave
1.44 MB
220_030_03
Carbohydrates in Dutch candy
0.63 MB
220_031_06
Sucralose
2.18 MB
220_032_03
Sugars in beer
2.33 MB
220_033_01
Sugars and sucralose
2.39 MB

Prebiotics food additives

 

Rev
Name
Size
Download
220_020_05
TGOS in food products
2.02 MB
220_021_09
Profiling of fructooligosaccharides
1.81 MB
220_022_04
Fructans in infant formula
1.13 MB
220_027_03
Analysis of maltodextrin in syrups
1.46 MB

Lactose free products

 

Rev
Name
Size
Download
220_009_16
Lactose-free products
2.48 MB
220_018_05
Lactose in dairy and meat products
1.61 MB

Carbohydrates in plants

 

Rev
Name
Size
Download
220_004_07
Carbohydrates in plants
1.00 MB

Artificial sweeteners

 

Rev
Name
Size
Download
220_013_08
Sugar alcohols
1.34 MB
220_031_06
Sucralose
2.18 MB
220_033_01
Sugars and sucralose
2.39 MB

Compositional analysis of glycoproteins

 

Rev
Name
Size
Download
220_024_04
Monosaccharides in glycoproteins
1.96 MB
220_026_08
Analysis of intact N-Glycans by HPAEC-PADMS
1.07 MB
220_034_01
Sialic acids in glycoproteins
1.69 MB