Welcome to our Knowledge Base
Poor reproducibility – autosampler issues
Applies to
ALEXYS system, autosampler
Summary
Poor injection reproducibility caused by autosampler.
Cause
- Air in flow path
- Leaking syringe
- Worn-out rotor seal
- Dead volumes in flow path
- Incorrect user defined program
Diagnosis and Remedy
When a user program is used, check the reproducibility with a standard injection procedure (full loop injections). If the problem disappears, then optimize the user defined injection program until reproducible results are obtained. Follow the trouble-shoot instructions in the diagram below.
