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Poor reproducibility – autosampler issues

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Applies to

ALEXYS system, autosampler


Poor injection reproducibility caused by autosampler.


  1. Air in flow path
  2. Leaking syringe
  3. Worn-out rotor seal
  4. Dead volumes in flow path
  5. Incorrect user defined program

Diagnosis and Remedy

When a user program is used, check the reproducibility with a standard injection procedure (full loop injections). If the problem disappears, then optimize the user defined injection program until reproducible results are obtained. Follow the trouble-shoot instructions in the diagram below.


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