In drug and xenobiotic metabolism the metabolites are formed instantaneously in the electrochemical cell mimicking the enzymatic biotransformation of the Cytochrome P450 reactions that usually take place in the liver (Phase I reaction). Hereby Electrochemistry becomes a truly biomimetic tool for enzymatic REDOX reactions.
Significant time and cost saving are possible compared to days or weeks using the traditional in-vitro (e.g., microsomes) and/or in-vivo methods (e.g., rodents, human, etc.). No cumbersome isolation form biological matrices (e.g., urine, plasma) is required and there is no longer risk of adsorption/binding to cells or other biological constituents. By adding glutathione after the electrochemical cell, adduct formation (phase II reaction) can be simulated and detected on-line by MS. Further advantages are: generation of intermediates and short-lived, unstable metabolites, no test animals (rodents), no biohazard risk (human liver microsomes).
Phase I metabolism of Tetrazepam
In only a few minutes all major metabolites of Tetrazepam could be generated and identified by on-line EC/LC/MS. Substantial time savings result using ROXY EC for biomimetic oxidation compared to microsomal incubation followed by metabolite extraction or the even more daunting and cumbersome isolation from urine. Ref: A. Baumann, J of Chrom. A, 1216 (2009) 3192
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