The µ-PrepCell2.0 can be used successfully in flow chemistry for the synthesis of metabolites and other REDOX species (e.g., intermediates). Typical quantities are in the µg range / minute. Collecting the products over 1 hour or more, result in sufficient amounts for identification by modern NMR. Huge time savings can be realized compared to syntehsis by wet chemistry or the expensive and often non-specific enzymatic approaches.
Application of a square wave pulse is beneficial for metabolite synthesis. The main advantage of applying a pulse is a stable current over a long period of time and a constant synthesis yield. The electrode surface is thereby continuously reactivated, reducing adsorption or fouling, meanwhile higher sample concentration can be introduced into the cell.
A) Overlay of 8 mass spectra acquired over 3.5 hrs, illustrating the excellent stability of synthesis of the 3 Verapamil metabolites: m/z 196, m/z 291 and m/z 441. (B) Applied square wave pulse settings for synthesis of Verapamil metabolites with E1 800 mV and E2 400 mV and durations of appr. 2 and 1 s, respectively.
After collection and purification by HPLC the individual metabolites can be analyzed by NMR and/or MS and used as reference material.
See References below or contact Antec Scientific at email@example.com
23812883 - EC-SPE-stripline-NMR analysis of reactive products: a feasibility study.
Falck D, Oosthoek-de Vries AJ, Kolkman A, Lingeman H, Honing M, Wijmenga SS, Kentgens AP, Niessen WM.; Anal Bioanal Chem. 2013 Aug;405(21):6711-20. doi: 10.1007/s00216-013-7158-9. Epub 2013 Jun 30.
22971210 - Combination of electrochemistry and nuclear magnetic resonance spectroscopy for metabolism studies.
Simon H, Melles D, Jacquoilleot S, Sanderson P, Zazzeroni R, Karst U.; Anal Chem. 2012 Oct 16;84(20):8777-82. doi: 10.1021/ac302152a. Epub 2012 Sep 25.
26116769 - Preparing the key metabolite of Z-ligustilide in vivo by a specific electrochemical reaction
F Duan, W Xu, J Liu, Z Jia, K Chen; Journal of Separation Science (2018) 41: 2799-2807
26116770 - Prediction of biotransformation products of the fungicide fluopyram by electrochemistry coupled online to liquid chromatography-mass spectrometry and comparison …
TF Mekonnen, U Panne, M Koch ; Analytical and Bioanalytical Chemistry (2018) 410: 2607-2617
26116771 - Electrochemical simulation of triclosan metabolism and toxicological evaluation
L Zhu, Y Shao, H Xiao, B Santiago-Schübel; Science of the Total Environment (2018) 622–623: 1193-1201
26116773 - Electrochemical oxidation of fluoroquinolone antibiotics: Mechanism, residual antibacterial activity and toxicity change
L Zhu, B Santiago-Schübel, H Xiao, H Hollert; Water Research (2016) 102: 52-62
26116774 - KAE609 (Cipargamin), a new spiroindolone agent for the treatment of malaria: Evaluation of the Absorption, Distribution, Metabolism and Excretion of a single oral 300 …
SEW Huskey, C Zhu, A Fredenhagen, J Kuhnol; Drug Metabolism and Disposition (2016) DOI: https://doi.org/10.1124/dmd.115.069187
26116775 - … pharmaceutical oxidation products using electrochemistry: a systematic study of N-dealkylation reactions of fesoterodine using a commercially available synthesis cell
S Torres, R Brown, R Szucs, JM Hawkins; Org. Process Res. Dev. (2015) 19: 1596-1603
26116776 - An efficient laboratory workflow for environmental risk assessment of organic chemicals
L Zhu, B Santiago-Schübel, H Xiao, B Thiele, Z Zhu; Chemosphere (2015) 131: 34-40
00216-013-7021 - Generation of statin drug metabolites through electrochemical and enzymatic oxidations
Smriti Khera, Na Hu; Analytical and Bioanalytical Chemistry, July 2013, Volume 405, Issue 18, pp 6009–601
See our support pages for a full list of recent literature