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Hydrogen peroxide

hydrogen peroxide




Hydrogen peroxide is a strong oxidizing agent and disinfectant used in a wide variety of applications, such as chemical synthesis, propellant (space rockets), water treatment, food processing, cosmetics, personal and health care products [1].
In this application note an analytical method is presented for the measurement of hydrogen peroxide in liquid samples using the DECADE Elite electrochemical detector and SenCell. The method is based on separation by High Performance Anion Exchange Chromatography followed by Pulsed Amperometric Detection (HPAE-PAD) on a gold working electrode.  The use of a narrow-bore HPAE column with 4µm particle size, allowed the separation of  hydrogen peroxide in less than 3 min in combination with a four-fold reduction of mobile phase usage.
A commercially available whitening toothpaste, containing peroxide as bleaching agent, was analyzed as an example to demonstrate the applicability of the method to real samples.

Application Notes

System Configuration

For analysis of low concentrations of hydrogen peroxide we use the ALEXYS Analyzer. For best sensitivity we use the DECADE Elite with Sencell in combination with a new 4-step pulse for detection. This new PAD waveform minimizes the wear of the gold electrode. It increases the flow cell life-time and has less maintenance.


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