Tutorials on HPLC/ECD

This webcast from Nov 4, 2021, covers all aspects to successfully perform HPLC with Electrochemical Detection (ECD). Detection principle, method development and optimization are discussed.

Key Learning Objectives
• Principles of electrochemical detection (ECD)
• How to improve sensitivity and robustness
• Learn how easy HPLC with state-of-the-art ECD has become

This video explains the basic principle of electrochemical detection for HPLC. When an electrochemically active substance elutes from the column, it enters the flow cell and hits the electrode surface. On the surface the electrochemical reaction takes place, the electrons are transferred and a peak appears in the chromatogram.

The DECADE Elite is a fully featured electrochemical detector for HPLC. It can operate in the DC, pulse and scan mode, and it can control up to 4 flow cells simultaneously. It is the most sensitive detector with extremely low limits of detection. This video (9 min) explains all about its special features.

The flow cell is the heart of the electrochemical detector, it is where the electrochemical reaction takes place. We have different flow cells with specific benefits for different applications. This video (5 min) explains all about the electrodes in the cell and specific features of the different models.

When developing the ALEXYS Neurotransmitter Analyzer our application developers focussed on a number of things: small samples, sensitivity, separation, selectivity and speed of analysis. Our goal is to get as much information as possible from 1 drop of dialysate. Bottlenecks can be found everywhere in the system from sampling to data handling. There is always a bottleneck somewhere in getting better results. This video (10 min) explains about the elimination of bottlenecks to get better results.