Login | Register

Glutathione and disulfides

Chromatogram Glutathione / Disulfides

ALEXYS Disulfides Analyzer





The Disulfides Analyzer has been developed for the analysis of (di-) sulfides such as (di-)glutathione, cystine, cysteine, homocystine, and homocysteine. The method applies a Flexcell with a Boron Doped Diamond working electrode for detection of mono and disulfides. Detection limits are below 0.1 µmol/L for most (di-)sulfides. In an alternative setup a dual flowcell configuration is used with about 10 times better detection limit. The method uses an additional Flexcell for pre-reduction of di-thiols, a second cell is used for detection. The chromatogram on the right shows the analysis of 2 µL, 1 µmol/L cystine (1), cysteine (2), glutathione (3), homocysteine (4) and diglutathione (5). If only the mono-sulfides are of interest, the Flexcell can be equipped with a Gold working electrode. This offers additional selectivity, and the lower working potential results in a better detection limit as well (10 nmol/L).

Application Notes

System Configuration

For the analysis of disulfides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode. The ALEXYS system comprises the following parts:

  • AS 110 autosampler, cool
  • LC 110S HPLC pump
  • OR 110 organiser rack ALEXYS for disulfides
  • Clarity data acquisition software
  • DECADE Elite electrochemical detector SCC
  • Flexcell with BDD WE, sb REF

or (high sensitivity DCC method only):

  • DECADE Elite electrochemical detector DCC
  • 2 Flexcells with GC and Gold WE, HyREF

For more details see our product catalogue.


Part no Description
180.0068D ALEXYS Disulfides Analyzer (2 flow cells, high sensitivity)
180.0069C ALEXYS Disulfides Analyzer using 1 Flexcell, BDD electrode