Login | Register

GABA and Glutamate

ALEXYS Neurotransmitter Analyzer

ALEXYS Neurotransmitter Analyzer



The ALEXYS Neurotransmitter Analyzer is a modular system that can be customized for the analysis of specific neurotransmitters. A sensitive method has been developed for the analysis of the amino acid neurotransmitters GABA and glutamate, as well as histamine and several other LNAA's (large neutral amino acids). It has a fully automated OPA derivatisation procedure using an AS 110 user program. Late eluting peaks from the OPA reagent are conveniently removed by an automated wash step. GABA/Glu analyzer can be down-scaled to a sample volume < 10 µL.

Application Notes

Application Notebook

  • 213_070_06 - Application Notebook Neurotransmitter

System configuration

For the analysis of GABA and Glutamate we selected the SenCell. This cell not only has an excellent reproducibility but also the best possible detection sensitivity. We routinely inject 5-20 µL, we selected our AS 110 autosampler (standard, cool). We use a 1 mm ID column at 50 µL/min as a standard. A second pump is used for post-run washing step of late eluting substances with high modifier.

The ALEXYS system comprises the following parts:

  • AS 110 autosampler, standard cool
  • P6.1L HPLC pump (2x)
  • Clarity data acquisition software
  • DECADE Elite electrochemical detector, SCC (single channel)
  • SenCell with GC WE, salt bridge

 GABA and Glutamate:

Part no Description
180.0091W ALEXYS Neurotransmitter Analyzer
191.0055UL AS 110 UHPLC cool 6p, LAN
180.0602 LC step-gradient upgrade
180.0504 ALEXYS GABA/Glu kit

GABA and Glu Analyzer