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Choline and Acetylcholine

Choline and acetylcholine

Basal level rat microdialysate, 1 nmole/L ACh.

ALEXYS Neurotransmitter Analyzer

 

 

 

 

 

The ALEXYS Neurotransmitter Analyzer can be used for fast and sensitive detection of basal acetylcholine (ACh) levels in microdialysis samples. Analysis of ACh by HPLC-ECD is based on an ion-pairing separation, followed by post-column enzymatic conversion to hydrogen peroxide with acetylcholinesterase (AChE) and choline oxidase (ChO). Both enzymes are covalently bound to a stationary phase in an immobilized enzyme reactor (IMER). After conversion, the hydrogen peroxide is detected using the Elite electrochemical detector with Sencell on a Pt electrode. The method is optimised for analysis of small sample volumes at low nanomolar detection limit.


Application Notes

Application Notebook

  • 213_070_05 - Application Notebook Neurotransmitter

System configuration

The ALEXYS system uses a DECADE Elite and SenCell in combination with the ACh/Ch assay kit. The ALEXYS system comprises the following parts:

  • AS 110 autosampler, cool, micro
  • LC 110S HPLC pump
  • OR 110 organiser rack ACH analyzer
  • Clarity data acquisition software
  • DECADE Elite SCC
  • SenCell, Pt WE, HyREF 

 For more details see product catalogue.

 

Part no Description
180.0091E ALEXYS Neurotransmitter  BP, 1 ch
191.0035U AS 110 autosampler UHPLC cool 6p
180.0505 Acetylcholine kit
250.3532 AChE/ChOx post column-IMER