Basal level rat microdialysate, 1 nmole/L ACh.
The ALEXYS Neurotransmitter Analyzer can be used for fast and sensitive detection of basal acetylcholine (ACh) levels in microdialysis samples. Analysis of ACh by HPLC-ECD is based on an ion-pairing separation, followed by post-column enzymatic conversion to hydrogen peroxide with acetylcholinesterase (AChE) and choline oxidase (ChO). Both enzymes are covalently bound to a stationary phase in an immobilized enzyme reactor (IMER). After conversion, the hydrogen peroxide is detected using the Elite electrochemical detector with Sencell on a Pt electrode. The method is optimised for analysis of small sample volumes at low nanomolar detection limit.
The ALEXYS system uses a DECADE Elite and SenCell in combination with the ACh/Ch assay kit. The ALEXYS system comprises the following parts:
For more details see product catalogue.
|180.0091E||ALEXYS Neurotransmitter BP, 1 ch|
|191.0035U||AS 110 autosampler UHPLC cool 6p|
|250.3532||AChE/ChOx post column-IMER|