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Choline and Acetylcholine

ALEXYS Neurotransmitter Analyzer

 

The ALEXYS Neurotransmitter Analyzer can be used for fast and sensitive detection of basal acetylcholine (ACh) levels in microdialysis samples. Analysis of ACh by HPLC-ECD is based on an ion-pairing separation, followed by post-column enzymatic conversion to hydrogen peroxide with acetylcholinesterase (AChE) and choline oxidase (ChO). Both enzymes are covalently bound to a stationary phase in an immobilized enzyme reactor (IMER). After conversion, the hydrogen peroxide is detected using the Elite electrochemical detector with Sencell on a Pt electrode. The method is optimised for analysis of small sample volumes at low nanomolar detection limit.


Application Notes

Application Notebook

  • 213_070_06 - Application Notebook Neurotransmitter

System configuration

The ALEXYS system uses a DECADE Elite and SenCell in combination with the ACh/Ch assay kit. The ALEXYS system comprises the following parts:

  • AS 110 autosampler, cool, micro
  • P6.1L HPLC pumpACH analyzer
  • Clarity data acquisition software
  • DECADE Elite SCC
  • SenCell, Pt WE, HyREF 

 For more details see product catalogue.

 

Part no Description
180.0091W ALEXYS Neurotransmitter  BP, 1 ch
191.0055UL AS 110 autosampler UHPLC cool 6p
180.0505 Acetylcholine kit
250.3532 AChE/ChOx post column-IMER