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Iodide in urine


ALEXYS Anions Analyzer




A method for the determination of iodide is developed using electrochemical detection (ECD) with a silver working electrode (WE). The method consists of reversed phase chromatography using a ion pair and phosphate/citrate buffer at pH 6.5, followed by amperometric detection at 0.15 V. The silver electrode is extremely selective towards iodide, which is detected at a potential close to zero. As a result the sample prep is easy, only a dilution and filtration step of the urine samples. The chromatogram shows a triplicate analysis of a urine sample containing about 2 µmol/L iodide.

Application Notes

System configuration

For the analysis of Iodide we selected the Flexcell with silver working electrode. The cell as a virtually maintenance free HyREF reference electrode.

The ALEXYS system comprises the following parts:

  • AS 110 autosampler, cool
  • P6.1L HPLC pump
  • DECADE Lite electrochemical detector
  • Flexcell with Ag WE and HyREF
  • LC connection kit
  • USB connection kit, 3 portALEXYS with DECADE Elite
  • Clarity data acquisition software



Part no Description
180.0095W ALEXYS Iodide Analyzer