Glutathione and disulfides
ALEXYS Disulfides Analyzer
- Both mono- and disulfides in one run
- Single flow cell with diamond electrode, or
- Dual flow cell for selectivity with gold electrode
- Detection limits 2-10 (gold) or 25-100 nmol/L (diamond)
The Disulfides Analyzer has been developed for the analysis of (di-) sulfides such as (di-)glutathione, cystine, cysteine, homocystine, and homocysteine. The method applies a Flexcell with a Boron Doped Diamond working electrode for detection of mono and disulfides. Detection limits are below 0.1 µmol/L for most (di-)sulfides. In an alternative setup a dual flowcell configuration is used with about 10 times better detection limit. The method uses an additional Flexcell for pre-reduction of di-thiols, a second cell is used for detection. The chromatogram on the right shows the analysis of 2 µL, 1 µmol/L cystine (1), cysteine (2), glutathione (3), homocysteine (4) and diglutathione (5). If only the mono-sulfides are of interest, the Flexcell can be equipped with a Gold working electrode. This offers additional selectivity, and the lower working potential results in a better detection limit as well (10 nmol/L).
Application Notes
- 211_004_06 - Glutathione and Other Disulfides
- 211_005_02 - Aminothiols, Glutathione and disulfides
Posters
- 221_010_01 - Glutathione and other disulfides
System Configuration
For the analysis of disulfides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode. The ALEXYS system comprises the following parts:
- AS 110 autosampler, cool
- P6.1L HPLC pump
- Clarity data acquisition software
- DECADE Elite electrochemical detector SCC
- Flexcell with BDD WE, sb REF
or (high sensitivity DCC method only):
- DECADE Elite electrochemical detector DCC
- 2 Flexcells with GC and Gold WE, HyREF
For more details see our product catalogue.
| Part no | Description |
| 180.0068W | ALEXYS Disulfides Analyzer (2 flow cells, high sensitivity) |
| 180.0069W | ALEXYS Disulfides Analyzer using 1 Flexcell, BDD electrode |