Glutathione and disulfides
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ALEXYS Disulfides Analyzer
- Both mono- and disulfides in one run
- Single flow cell with diamond electrode, or
- Dual flow cell for selectivity with gold electrode
- Detection limits 2-10 (gold) or 25-100 nmol/L (diamond)
The Disulfides Analyzer has been developed for the analysis of (di-) sulfides such as (di-)glutathione, cystine, cysteine, homocystine, and homocysteine. The method applies a Flexcell with a Boron Doped Diamond working electrode for detection of mono and disulfides. Detection limits are below 0.1 µmol/L for most (di-)sulfides. In an alternative setup a dual flowcell configuration is used with about 10 times better detection limit. The method uses an additional Flexcell for pre-reduction of di-thiols, a second cell is used for detection. The chromatogram on the right shows the analysis of 2 µL, 1 µmol/L cystine (1), cysteine (2), glutathione (3), homocysteine (4) and diglutathione (5). If only the mono-sulfides are of interest, the Flexcell can be equipped with a Gold working electrode. This offers additional selectivity, and the lower working potential results in a better detection limit as well (10 nmol/L).
Application Notes
- 211_004_06 - Glutathione and other Disulfides
- 211_005_02 - Aminothiols, Glutathione and Disulfides
Posters
- 221_020_02 - Disulfides with Magic Diamond Electrode
- 221_003_02 - Homocysteine in plasma
System Configuration
For the analysis of disulfides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode. The ALEXYS system comprises the following parts:
- AS 110 autosampler, cool
- LC 110S HPLC pump
- OR 110 organiser rack
- Clarity data acquisition software
- DECADE Elite electrochemical detector SCC
- Flexcell with BDD WE, sb REF
or (high sensitivity DCC method only):
- DECADE Elite electrochemical detector DCC
- 2 Flexcells with GC and Gold WE, HyREF
For more details see our product catalogue.
| Part no | Description |
| 180.0068D | ALEXYS Disulfides Analyzer (2 flow cells, high sensitivity) |
| 180.0069C | ALEXYS Disulfides Analyzer using 1 Flexcell, BDD electrode |