The Disulfides Analyzer has been developed for the analysis of (di-) sulfides such as (di-)glutathione, cystine, cysteine, homocystine, and homocysteine. The method applies a Flexcell with a Boron Doped Diamond working electrode for detection of mono and disulfides. Detection limits are below 0.1 µmol/L for most (di-)sulfides. In an alternative setup a dual flowcell configuration is used with about 10 times better detection limit. The method uses an additional Flexcell for pre-reduction of di-thiols, a second cell is used for detection. The chromatogram on the right shows the analysis of 2 µL, 1 µmol/L cystine (1), cysteine (2), glutathione (3), homocysteine (4) and diglutathione (5). If only the mono-sulfides are of interest, the Flexcell can be equipped with a Gold working electrode. This offers additional selectivity, and the lower working potential results in a better detection limit as well (10 nmol/L).
For the analysis of disulfides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode. The ALEXYS system comprises the following parts:
or (high sensitivity DCC method only):
For more details see our product catalogue.
|180.0068D||ALEXYS Disulfides Analyzer (2 flow cells, high sensitivity)
|180.0069C||ALEXYS Disulfides Analyzer using 1 Flexcell, BDD electrode