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Azithromycin, Erythromycin and other Macrolide Antibiotics

Analysis of Azithromycin

ALEXYS Azithromycin Analyzer





The macrolides are a group of antibiotics whose activity stems from the presence of a macrolide ring, a large macrocyclic lactone ring to which one or more deoxy sugars may be attached.

The antimicrobial spectrum of macrolides is slightly wider than that of penicillin, and, therefore, macrolides are a common substitute for patients with a penicillin allergy. Azithromycin (Azi) is a semisynthetic macrolide antibiotic. It is effective against a wide variety of bacteria organisms, such as Hemophilus influenzae, Streptococcus pneumoniae, Staphylococcus aureus, and many others. Azithromycin is used to treat bacterial infections such as bronchitis, pneumonia, sexually transmitted diseases (STD) and infections of the ears, lungs, skin, and throat.

Other macrolide antibiotics that can be reproducibly analysed with the ALEXYS Analyzer are Clarithromycin, Erythromycin, Roxithromycin.

Application Notes

System configuration

For the analysis of Azythromycin we selected the SenCell. This cell not only has an excellent reproducibility but also the best possible detection sensitivity. A Reactor was used as pre-oxidation screening cell to meet USP requirements. The ALG is a USP L49 type of column.

The ALEXYS system comprises the following parts:

  • AS 110 autosampler, cool
  • P6.1L HPLC pump
  • Clarity data acquisition software
  • DECADE Elite electrochemical detector, DCC
  • SenCell with GC WE, sb REF ALEXYS with DECADE Elite
  • Flexcell with GC WE and HyREF



Part no Description
180.0086W ALEXYS Azithromycin Analyzer, USP