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AS 110

µ-PrepCell™ 2.0 – the Perfect Workhorse



The new µ-PrepCell 2.0 (µPC 2.0) is specifically designed for highest robustness and ease of use. The entire cell is made of a fully inert polymeric material. The inlet block contains additional conductive carbon, to serve as Auxiliary (AUX) electrode. In addition the Reference electrode (REF) is positioned in a reference chamber, resulting in overall improved signal stability and reproducibility. In practice the new µPC 2.0 has distinguished advantages in both Oxidative and Reductive mode.

Compounds passing through the flow cell are instantaneously oxidized or reduced making the cell ideally suited for infusion EC/MS, HDX/MS or LC/EC/MS. The cell can be used in various application areas such as: drug metabolism (generation of metabolites), protein chemistry (reduction of disulfide bonds, H/D exchange, etc.), environmental degradation (generation of transformation products), stability testing of drugs, nutrients, etc. In continuous infusion mode over several hours, the cell can be employed for micro preparative work, generating µg quantities of REDOX products.


Schematics µ-PrepCell 2.0 set-up:

(A) Direct infusion mode EC/MS used for oxidation/reduction of target compound.

(B) Typical EC/LC/MS set-up used in HDX/MS with high pressure uPC. 

(C) EC after HPLC separation, i.e., LC/EC/MS, used in bottom-up proteomics, REDOX on complex samples. 

Typical flow rates 20 to 200 μL/min.






Like the ReactorCell™, the µPc 2.0 is based on a thin-layer flow cell concept, comprising of a very smooth working electrode surface over which the sample is flowing. This results in almost zero sample adsorption, unlike porous flow through cells which are prone to adsorption and carryover. The cell can be disassembled within a few seconds for easy cleaning or exchange of working electrode, e.g. making the µPC 2.0 the ideal workhorse. The cell is used with the ROXY Potentiostat or integrated in the ROXY EC or ROXY EC/LC system, respectively. Flow rates, potentials, events programming, recording of voltammograms etc., are fully controlled via the Dialogue™ Elite software.

Large Variety of Working Electrodes

For maximum selectivity, yield and wide applicability, a large variety of electrode materials are available such as Glassy Carbon (GC) and Magic Diamond™ (BDD: Boron Doped Diamond), Au and Pt. For reduction of Disulfide bonds in proteins/peptides the new TiBlue™ electrode is available.  For more details, see specs




Oxidative Mode

The new conductive polymeric inlet block results in significant higher selectivity by suppressing unwanted reduction reactions when working under oxidative conditions (Ox only). The electrochemical oxidation of Amodiaquine was used as example. Using the new uPC 2.0, only the 3 oxidation products were formed without any of the reduction products.
For more information, see brochure µ-PrepCell 2.0. 

Another advantage is the larger potential working range as can be seen from the scanning voltammogram of Dopamine. With the new cell, a significant larger working range is possible. The previous cell showed a current overload at around +1.5 V, meanwhile the new cell showed no current overflow up to +2 V and much better reproducibility.

Scanning Voltammetry of Dopamine

Comparison previous (left) and new µ-PrepCell 2.0 (right).ROXY Potentiostat. Scan mode from -300 mV to +1500 mV for previous cell (left) and -300 mV to +2000 mV for the new µPC 2.0 (right); scan rate 20mV/s, Glassy Carbon as WE.

Reductive Mode

In reductive mode the new µ-PrepCell 2.0 allows to work in simple DC mode for the cleavage of disulfide bonds in proteins/peptides. A potential of ca. -2.0 V is sufficient to reduce selectively the S-S bonds, making the use of square wave pulses obsolete. In combination with the new TiBlue™ electrode a much more stable and reproducible reduction is achieved. For more details, see brochure µ-PrepCell 2.0.

Long Term Stability and Reproducibility

Reduction of Insulin over 14 hrs in continuous flow mode using the new µPC 2.0 and TiBlue electrode. Flow rate 20 µL/min, DC mode (E= -2.0V). The sample (5μg/mL insulin in 1% formic acid, 10% acetonitrile, 89% water) was injected every 10 min to check reduction efficiency. Grey dots: intact insulin (m/z 956); blue dots: chain B (m/z 681) obtained after reduction of the two interchain disulfide bonds. 

TiBlue™ Electrode 

Efficient reduction of disulfide bonds in proteins/peptides


The electrodes are made by a proprietary surface treatment of a Titanium alloy, resulting in a blue-coloured crystalline TiO2 layer. The electrodes can be used both sided and are disposable. 

Product Flyers



Part no Description
204.4300T μ-PrepCell Ti, consisting of:
μ-PrepCell with mounting brace, spacers, reference electrode (HyRef) and 
Ti based working electrode for S-S bond reduction. 
204.4310 μ-PrepCell 2.0 GC/BDD, consisting of:
μ-PrepCell 2.0 with mounting brace, spacers, reference electrode (HyRef) and
working electrodes: Glassy Carbon (GC), Magic Diamond (BDD), 1 each.
204.4302 µ-PrepCell high pressure, Ti, consisting of:
μ-PrepCell high pressure with mounting brace, spacers, reference electrode (HyRef) and Ti based working electrode for S-S bond reduction.
204.4312 µ-PrepCell 2.0 TiBlue, consisting of:
μ-PrepCell 2.0 with mounting brace, spacers, reference electrode (HyRef) and
1 x mounted TiBlue and 1 x blister with 2 TiBlue electrodes 
204.5007 Glassy Carbon (GC) working electrode
204.5050 Magic Diamond (BDD) working electrode
204.5010 Titanium (Ti) based working electrode (S-S bond reduction)
204.5010A TiBlue working electrode, blister with set of 2 electrodes
204.5022 Platinum (Pt) working electrode
Gold (Au) working eletrode
204.0913 μ-PrepCell HyREF
Spare Parts
204.0501 O-ring inlet block, Silicone, 5pcs
204.0503 O-ring REF electrode, Silicone, 5pcs
204.0505 O-ring inlet block, Kalrez, 5pcs
204.2217 Spacer 50 µm
204.2218 Spacer 100 µm

A number support tools are available on this website:

ROXY installation with µ-PrepCell in 2 minutes: click to play or download the movie (right click and "save link as..." if video does not start). If your player does not recognize the format, download the free player from VLC (Windows or Mac).