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µ-PrepCell

The µ-PrepCell™ is the perfect workhorse to perform electrochemical reactions with high conversion efficiencies at flow rates up to 200 µl/min. Two versions are available. The µ-PrepCell 2.0 is the best choice for oxidative metabolism and degradation studies, while the µ-PrepCell SS is a dedicated cell for efficient disulfide bond reduction in peptides and proteins.

Oxidations: µ-PrepCell 2.0

For oxidative reactions the µ-PrepCell 2.0  is the cell of choice providing highest selectivity and high yield by the availability of different working electrodes such as Glassy Carbon (GC), Magic Diamond™ (BDD: Boron Doped Diamond), Au and Pt. The large working potential of 0-4V by using BDD as working electrode is another benefit of the cell-electrode assembly that allows generating .OH radicals the most powerful oxidizing agent. The cell can be disassembled within a few seconds for easy cleaning or exchange of working electrode, making the µ-PrepCell 2.0 the ideal workhorse for oxidative reactions.

Reductions: µ-PrepCell SS

For the reduction of disulfide bonds in peptides/proteins including mAbs the newly developed µ-PrepCell SS is the cell of choice. Unlike the chemical reduction using harsh reagents such as DTT or TECP that often interfere with the LC-MS analysis, or costly enzymes, the on-line electrochemical reduction is a real green approach resulting in superior characterization of proteins in top-down and bottom-up proteomics including
HDX-MS. 

 

 

µ-PrepCell

 

The µ-PrepCell™ is the perfect workhorse to perform electrochemical reactions with high conversion efficiencies at flow rates up to 200 µl/min. Two versions are available. The µ-PrepCell 2.0 is the best choice for oxidative metabolomics and degradation studies, while the µ-PrepCell SS is a dedicated cell for efficient disulfide bond reduction in peptides and proteins.

Oxidations: µ-PrepCell 2.0

  • Robust and reproducible oxidations
  • Fast generation of drug-metabolites, and -degradants
  • Large working range (potential)
  • Excellent correlation with metabolic & degradation pathway

For oxidative reactions the µ-PrepCell 2.0  is the cell of choice providing highest selectivity and high yield by the availability of different working electrodes such as Glassy Carbon (GC), Magic Diamond™ (BDD: Boron Doped Diamond), Au and Pt. The large working potential of
0-4V by using BDD as working electrode is another benefit of the cell-electrode assembly that allows generating .OH radicals the most powerful oxidizing agent.
The cell can be disassembled within a few seconds for easy cleaning or exchange of working electrode, making the µ-PrepCell 2.0 the ideal workhorse for oxidative reactions.

Tekstvak:  Reductions: µ-PrepCell SS

  • Fast and efficient reduction of S-S bonds
  • Robust over several days of operation
  • Chemical free, no interfering DDT, TCEP
  • Superior peptide sequencing and S-S bond assignment

For the reduction of disulfide bonds in peptides/proteins including mAbs the newly developed µ-PrepCell SS is the cell of choice. Unlike the chemical reduction using harsh reagents such as DDT or TECP that often interfere with the LC-MS analysis, or costly enzymes, the on-line electrochemical reduction is a real green approach resulting in superior characterization of proteins in top-down and bottom-up proteomics including
HDX-MS.

 


Product Flyers

Manuals

 

Part no Description
204.4304 μ-PrepCell SS, consisting of:
μ-PrepCell with mounting bracket, cell cable, spacers, Ti working electrode (inlet block) and
Pt counter electrode for disulfide (S-S) bond reduction. 
204.4310 μ-PrepCell 2.0 GC/BDD, consisting of:
μ-PrepCell 2.0 with mounting bracket, cell cable, spacers, Pd/H2 reference electrode (HyREF) and 
working electrodes: Glassy Carbon (GC), Magic Diamond (BDD), 1 each.

Electrodes
204.5007 Glassy Carbon (GC) working electrode
204.5050 Magic Diamond (BDD) working electrode
204.5022 Platinum (Pt) working/counter electrode
204.5027 Gold (Au) working electrode
204.0913 HyREF for µ-PrepCell
Spare parts
204.0501 O-ring inlet block, Silicone, 5pcs
204.0506 O-ring inlet block, Silicone, 5pcs
204.0503 O-ring REF electrode, Silicone, 5pcs
204.0505 O-ring inlet block, Kalrez, 5pcs
204.2217 Spacer 50 µm
204.2218 Spacer 100 µm

A number support tools are available on this website:

ROXY installation with µ-PrepCell in 2 minutes: click to play or download the movie (right click and "save link as..." if video does not start). If your player does not recognize the format, download the free player from VLC (Windows or Mac).