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3-Nitrotyrosine

ALEXYS 3-Nitrotyrosine Analyzernitrotyrosine

 

 

 

 

Peroxynitrite has been reported to react with L-tyrosine to produce 3-nitro-L-tyrosine (NO2-Tyr), which appears to be a suitable marker for ONOO- mediated tissue damage. The NO2-Tyr standards are analyzed in a dual cell configuration with a detection limit of 0.5 nmol/L. As conversion in the first flow cell increases with lowering the flow rate, a micro LC (1mm) system is required. The ALEXYS system is optimised for analysis of small sample volumes and best detection performance.


Application Notes

  • 213_026_01 - Compilation of Neuroscience Applications
  • 213_026_02 - Compilation of Neuroscience Applications

System configuration

For the analysis of nitrotyrosine we selected the Sencell. This cell not only has an excellent reproducibility but also the best possible detection sensitivity. We routinely inject 2-5 µL with a maximum sample use of only 10 µL. We selected our AS 110 autosampler together with a LC 110 pump. We use a 1 mm ID column at 50 µL/min as a standard.

The ALEXYS system comprises the following parts:

  • AS 110 autosampler, standard, cool
  • LC 110S HPLC pumpNTyr2
  • OR 110 organiser rack
  • Clarity data acquisition software
  • DECADE Elite DCC electrochemical detector 
  • SenCell with GC WE, ISAAC
  • Flexcell with GC WE, HyREF

 For more details see our product catalogue.

Part no Description
180.0072C ALEXYS Nitrotyrosine Analyzer

 

A list of literature references can be found elsewhere on our website. External sources are available as well, see for example these references in Google Scholar about the DECADE detectors.