ROXY EC/MS for mimicing natures redox reactions.
The on-line coupling of Electrochemistry (EC) with LC/MS has shown great potential for the investigation of drug metabolism. It offers fast electrochemical synthesis of drug metabolites for reliable quantification by mass spectrometry (MS) to be operated as a high throughput screening system. The system is suitable to mimic (synthesize) phase I metabolites within minutes, that otherwise are obtained by time consuming in vivo (laboratory animals) or in vitro experiments (isolated liver cells, microsomes).
A pre-injection on-line electrochemical cell has been used to mimic liver metabolism. Metabolites are instantaneously generated and analysed. Briefly, a syringe pump is used to flush xenobiotics through an electrochemical cell to fill an injection loop. The oxidation products are injected and analysed using HPLC-MS.
Comparing Fig. 1 and 2 clearly indicates that the electrochemical simulation leads to all metabolites also found in conventional method making it a perfect tool for Metabolism.
Mass traces of Tetrazepam metabolites formed in the electrochemical reaction cell of the ROXY™ EC/LC system.
Mass traces of Tetrazepam metabolites formed by the microsomal approach. Experiments were performed using pooled rat liver microsomes from male Sprague Dawley rats. Courtesy: A. Baumann, B. Schubert, H. Oberacher and U. Karst, University Münster, Münster, Germany.
For the simulation of oxidative metabolism Antec provides a number of electrode materials that are useful for the synthesis of metabolites. A platinum, and the new Magic Diamond™ electrodes are supplied. The system further comprises of the following parts:
|210.0080C||ROXY EC/LC system|