Aminoglycoside antibiotics
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ALEXYS Aminoglycoside Analyzers
- According US and European Pharmacopeia
- One Analyzer for all aminoglycosides
- FlexCell with easy exchangeable electrode
- Reproducible and robust analysis
The Aminoglycoside Analyzer can be used for the analysis of all type of aminoglycosides such as: Amikacin, Framycetin, Gentamicin, Kanamycin, Lincomycin, Neomycin, Spectinomycin, Streptomycin, Tobramycin, etc.
The system is dedicated to the analysis of these antibiotics in pharmaceutical formulations and bulk products. Aminoglycosides are manufactured by a fermentation process and the main constituents as well as impurities and by-products must be identified and quantified to fulfil USP and EP requirements. How to interprete the system suitability requirements has been described previously.
The system uses reversed phase chromatography with post column addition of sodium hydroxide for pulsed amperometric detection, except for the Kanamycin/Amikacin, which uses ion exchange chromatography. The supplied FlexCell with its easy exchangeable electrode guarantees for fast and user friendly operation.
Application Notes
- 217_015_12 - Amikacin and Kanamycin in Bulk Drugs
- 217_030_06 - Gentamicin EP USP
- 217_036_03 - Streptomycin according USP
- 217_013_12 - Gentamicin in Sulphate in Pharmaceutals
- 217_010_13 - Neomycin and Framycetin Sulfate in bulk drugs
- 217_031_05 - Netilmicin Sulfate according EP
- 217_019_08 - Spectinomycin and Lincomycin
- 217_018_10 - Spectinomycin in Pharmaceuticals
- 217_032_05 - Tobramycin according EP
- 217_014_09 - Tobramycin in Pharmaceuticals
- 217_000_00 - Adjustment to USP Monographs Flexibility and Limitations
Application Notebook
- 217_050_04 - Application Notebook Antibiotics
System configuration
For the analysis of aminoglycosides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode which is particularly useful in pulsed electrochemical detection. The electrode material tends to vanish over the years, in the process of pulsed amperometric detection. Exchanging an electrode disc only takes a few minutes. The HyREF is completely maintenance free even at pH 13 which is required for pulsed amperometric detection! The ALEXYS system comprises the following parts:
- AS 110 autosampler, cool
- P6.1L HPLC pump (2)
- Clarity data acquisition software
- DECADE Elite electrochemical detector
- Flexcell with Au WE, HyREF
Aminoglycosides 180.0050/56/59 require post-column addition of sodium hydroxide for detection. System 180.0058W is based on ion exchange chromatography at high pH (no post column addition). In addition to the firs
t mentioned list this system includes Helium-proof solvent bottles.
| Part no | Description |
| 180.0050W | ALEXYS Aminoglycosides Analyzer |
| 180.0056W | ALEXYS Gentamicin Analyzer |
| 180.0058W | ALEXYS Kanamycin/Amikacin Analyzer |
| 180.0059W | ALEXYS Spectinomycin/Lincomycin Analyzer |