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Lors de l’analyse compositionnelle des monosaccharides des glycoprot\u00e9ines \u00e0 l’aide du HPAEC-PAD, les acides amin\u00e9s et les petits peptides co\u00e9luent avec les hydrates de carbone d’int\u00e9r\u00eat, ce qui rend impossible une quantification correcte. Ces acides amin\u00e9s et ces petits peptides sont g\u00e9n\u00e9r\u00e9s lors de l’hydrolyse acide des glycoprot\u00e9ines. De plus, les acides amin\u00e9s peuvent contaminer la surface de l’\u00e9lectrode Au, ce qui peut entra\u00eener un encrassement et une perte de r\u00e9ponse, m\u00eame dans des conditions PAD. Pour \u00e9liminer l’interf\u00e9rence des acides amin\u00e9s et assurer une quantification pr\u00e9cise des monosaccharides, l’utilisation d’une colonne Amino Acid Trap (AAT) est fortement recommand\u00e9e. <\/p>\n
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[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb5%|5%|||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_image src=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2024\/11\/AAT-column.png\u00a0\u00bb title_text=\u00a0\u00bbColonne AAT\u00a0\u00bb _builder_version=\u00a0\u00bb4.27.3″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text _builder_version=\u00a0\u00bb4.27.3″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]Analyse des monosaccharides des glycoprot\u00e9ines avec et sans colonne Amino Acid Trap (AAT). Les pics interf\u00e9rents tels que la glutamine (Gln) et la lysine (Lys) sont pi\u00e9g\u00e9s et \u00e9luent plus tard au cours de l’\u00e9tape de lavage, voir la trace sup\u00e9rieure. <\/p>\n
\n<\/em>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb admin_label=\u00a0\u00bbDividerline\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb|0px|||false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%|||false|false\u00a0\u00bb locked=\u00a0\u00bboff\u00a0\u00bb collapsed=\u00a0\u00bbon\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_divider color=\u00a0\u00bb#dee1e6″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00a0\u00bb1″ admin_label=\u00a0\u00bbDownload Table\u00a0\u00bb _builder_version=\u00a0\u00bb4.27.2″ background_enable_color=\u00a0\u00bboff\u00a0\u00bb use_background_color_gradient=\u00a0\u00bbon\u00a0\u00bb background_color_gradient_stops=\u00a0\u00bb#f6f9fb 50%|#ffffff 100%\u00a0\u00bb background_color_gradient_start=\u00a0\u00bb#f6f9fb\u00a0\u00bb background_color_gradient_start_position=\u00a0\u00bb50%\u00a0\u00bb background_color_gradient_end=\u00a0\u00bb#ffffff\u00a0\u00bb max_width=\u00a0\u00bb100%\u00a0\u00bb max_width_tablet=\u00a0\u00bb\u00a0\u00bb max_width_phone=\u00a0\u00bb100%\u00a0\u00bb max_width_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb40px||40px||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_row _builder_version=\u00a0\u00bb4.27.2″ background_color=\u00a0\u00bbrgba(0,0,0,0)\u00a0\u00bb width_tablet=\u00a0\u00bb\u00a0\u00bb width_phone=\u00a0\u00bb\u00a0\u00bb width_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb max_width=\u00a0\u00bb80%\u00a0\u00bb max_width_tablet=\u00a0\u00bb\u00a0\u00bb max_width_phone=\u00a0\u00bb\u00a0\u00bb max_width_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_padding=\u00a0\u00bb||||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbShortcode of Table\u00a0\u00bb _builder_version=\u00a0\u00bb4.27.3″ background_color=\u00a0\u00bb#ffffff\u00a0\u00bb max_width=\u00a0\u00bb1200px\u00a0\u00bb custom_padding=\u00a0\u00bb20px|30px|30px|30px|false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|0px||0px|false|false\u00a0\u00bb custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb hover_enabled=\u00a0\u00bb0″ module_alignment_tablet=\u00a0\u00bb\u00a0\u00bb module_alignment_phone=\u00a0\u00bb\u00a0\u00bb module_alignment_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb box_shadow_style=\u00a0\u00bbpreset1″ box_shadow_blur=\u00a0\u00bb40px\u00a0\u00bb box_shadow_color=\u00a0\u00bbrgba(0,33,91,0.17)\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb sticky_enabled=\u00a0\u00bb0″]<\/p>\nBrochure et manuel d’utilisation<\/h3>\n