{"id":17772,"date":"2021-10-10T06:46:07","date_gmt":"2021-10-10T04:46:07","guid":{"rendered":"https:\/\/antecscientific.com\/techniques\/detection-amperometrique-pulsee\/"},"modified":"2024-12-09T11:12:03","modified_gmt":"2024-12-09T10:12:03","slug":"detection-amperometrique-pulsee","status":"publish","type":"page","link":"https:\/\/antecscientific.com\/fr\/produits\/techniques\/detection-amperometrique-pulsee\/","title":{"rendered":"D\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e"},"content":{"rendered":"

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Qu’est-ce que la d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e ?<\/h2>\n

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La d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e (PAD), est utilis\u00e9e dans l’analyse HPLC des hydrates de carbone et des substances apparent\u00e9es. \u00c0 un pH \u00e9lev\u00e9, les glucides peuvent \u00eatre oxyd\u00e9s et analys\u00e9s avec une grande sensibilit\u00e9. En utilisant la PAD, les conditions de mesure restent stables et reproductibles.<\/p>\n

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Comment fonctionne la d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e ?<\/h2>\n

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Dans la d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e (PAD), une s\u00e9quence rapide d’\u00e9tapes de potentiel est appliqu\u00e9e. Pendant une partie de cette \u00ab\u00a0s\u00e9quence d’impulsions\u00a0\u00bb, le signal est \u00e9chantillonn\u00e9. L’autre partie de la s\u00e9quence d’\u00e9tapes est constitu\u00e9e d’\u00e9tapes de nettoyage oxydatif et r\u00e9ducteur. Pendant ce nettoyage, l’\u00e9lectrode de travail en or est r\u00e9g\u00e9n\u00e9r\u00e9e. Le processus se r\u00e9p\u00e8te g\u00e9n\u00e9ralement toutes les demi-secondes environ. Cela garantit des conditions de d\u00e9tection stables et reproductibles.<\/p>\n

L’utilisation du PAD est vraiment n\u00e9cessaire, car la d\u00e9tection par la CE (oxydation) des glucides entra\u00eene des produits de r\u00e9action qui collent \u00e0 la surface de l’\u00e9lectrode. Cela contamine l’\u00e9lectrode et entra\u00eene une mauvaise reproductibilit\u00e9. En utilisant le PAD avec des \u00e9tapes de nettoyage, l’\u00e9lectrode reste propre et reproductible.<\/p>\n

Un effet secondaire du mode d’impulsion appliqu\u00e9 est que l’\u00e9lectrode en or est lentement consomm\u00e9e dans le processus. Apr\u00e8s une utilisation prolong\u00e9e (semaines, mois), on peut constater qu’un peu d’or a disparu. Les cellules de d\u00e9bit Antec utilisent une \u00e9lectrode en or massif, en cas de besoin la surface de l’\u00e9lectrode peut \u00eatre restaur\u00e9e en utilisant notre kit de polissage de m\u00e9tal. L’impulsion de d\u00e9tection traditionnelle \u00e0 3 \u00e9tapes s’use davantage que l’impulsion plus r\u00e9cente \u00e0 4 \u00e9tapes. Une FlexCell avec \u00e9lectrode de travail rempla\u00e7able peut \u00eatre utilis\u00e9e pour les deux. Un SenCell est recommand\u00e9 pour une impulsion \u00e0 4 \u00e9tapes seulement.<\/p>\n

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Potentiel de mesure E1 avec impulsion de nettoyage E2 – E4. La mesure se fait sur la seconde moiti\u00e9 de E1 (\u00e9chantillon t), lorsque le courant est stabilis\u00e9.<\/small><\/em><\/p>\n

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Pourquoi l’\u00e9limination du dioxyde de carbone est-elle n\u00e9cessaire dans les HPAEC?<\/h2>\n

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La chromatographie d’\u00e9change d’anions \u00e0 haute performance (HPAEC) est utilis\u00e9e pour l’analyse des hydrates de carbone par HPLC. Ce n’est qu’\u00e0 un pH<\/strong> tr\u00e8s \u00e9lev\u00e9<\/strong> que les hydrates de carbone sont des anions et peuvent \u00eatre s\u00e9par\u00e9s. Par cons\u00e9quent, seules quelques colonnes HPAEC peuvent \u00eatre utilis\u00e9es car elles doivent \u00eatre utilis\u00e9es \u00e0 un pH de 12 ou plus.<\/p>\n

\u00c0 un pH aussi \u00e9lev\u00e9, le dioxyde de carbone de l’air ambiant se dissout dans la phase mobile et forme des ions carbonate. La concentration de carbonate, qui augmente lentement, p\u00e9n\u00e8tre dans la colonne, occupe les groupements fonctionnels de la phase stationnaire et modifie les temps de r\u00e9tention des glucides. Il est donc tr\u00e8s important d’\u00e9liminer<\/strong> correctement le dioxyde de carbone<\/strong> de la phase mobile pour garantir des conditions de s\u00e9paration reproductibles.<\/p>\n

Le plateau d’\u00e9luant ALEXYS dispose de l’infrastructure n\u00e9cessaire pour maintenir les bouteilles de solvant sous une couverture d’azote (ou d’h\u00e9lium). Des bouchons \u00e9tanches au gaz, des tubes, des raccords et un manom\u00e8tre permettent d’ajuster le d\u00e9bit de gaz et de maintenir la consommation de gaz inerte \u00e0 un minimum absolu. <\/p>\n

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S\u00e9paration HPAEC et d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e des hydrates de carbone.<\/small><\/em><\/p>\n

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Qu’est-ce que la d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e ?La d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e (PAD), est utilis\u00e9e dans l’analyse HPLC des hydrates de carbone et des substances apparent\u00e9es. \u00c0 un pH \u00e9lev\u00e9, les glucides peuvent \u00eatre oxyd\u00e9s et analys\u00e9s avec une grande sensibilit\u00e9. En utilisant la PAD, les conditions de mesure restent stables et reproductibles.Comment fonctionne la d\u00e9tection amp\u00e9rom\u00e9trique […]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":16717,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-17772","page","type-page","status-publish","hentry"],"yoast_head":"\nD\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e - Antec Scientific<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/antecscientific.com\/fr\/produits\/techniques\/detection-amperometrique-pulsee\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"D\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e - Antec Scientific\" \/>\n<meta property=\"og:description\" content=\"Qu'est-ce que la d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e ?La d\u00e9tection amp\u00e9rom\u00e9trique puls\u00e9e (PAD), est utilis\u00e9e dans l'analyse HPLC des hydrates de carbone et des substances apparent\u00e9es. \u00c0 un pH \u00e9lev\u00e9, les glucides peuvent \u00eatre oxyd\u00e9s et analys\u00e9s avec une grande sensibilit\u00e9. 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