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L’analyseur de neurotransmetteurs ALEXYS peut \u00eatre utilis\u00e9 pour la d\u00e9tection rapide et sensible des niveaux basaux d’ac\u00e9tylcholine (ACh) dans les \u00e9chantillons de microdialyse. L’analyse de l’ACh par HPLC-ECD est bas\u00e9e sur une s\u00e9paration par appariement d’ions, suivie d’une conversion enzymatique post-colonne en peroxyde d’hydrog\u00e8ne avec l’ac\u00e9tylcholinest\u00e9rase (AChE) et la choline oxydase (ChO). Les deux enzymes sont li\u00e9es de mani\u00e8re covalente \u00e0 une phase stationnaire dans un r\u00e9acteur enzymatique immobilis\u00e9 (IMER). Apr\u00e8s conversion, le peroxyde d’hydrog\u00e8ne est d\u00e9tect\u00e9 \u00e0 l’aide du d\u00e9tecteur \u00e9lectrochimique DECADE Elite avec Sencell sur une \u00e9lectrode en Pt. La m\u00e9thode est optimis\u00e9e pour l’analyse de petits volumes d’\u00e9chantillons \u00e0 une faible limite de d\u00e9tection nanomolaire.<\/p>\n
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