{"id":16827,"date":"2020-08-30T07:27:57","date_gmt":"2020-08-30T07:27:57","guid":{"rendered":"https:\/\/antecscientific.com\/techniques\/electrochimie-avec-detection-ms\/"},"modified":"2023-05-08T15:37:41","modified_gmt":"2023-05-08T15:37:41","slug":"electrochimie-avec-detection-ms","status":"publish","type":"page","link":"https:\/\/antecscientific.com\/fr\/produits\/techniques\/electrochimie-avec-detection-ms\/","title":{"rendered":"\u00c9lectrochimie avec d\u00e9tection MS"},"content":{"rendered":"

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R\u00e9acteur pour EC\/MS<\/h2>\n

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Le couplage en ligne de l’\u00e9lectrochimie (EC) avec la spectrom\u00e9trie de masse (MS) a montr\u00e9 un grand potentiel pour l’\u00e9tude du m\u00e9tabolisme des m\u00e9dicaments dans les produits pharmaceutiques et pour l’\u00e9lucidation de la structure des produits biopharmaceutiques et des prot\u00e9ines. Comme son titre l’indique, cette technique se compose de deux parties. Tout d’abord, le r\u00e9acteur \u00e9lectrochimique o\u00f9 les produits ou m\u00e9tabolites sont form\u00e9s. Deuxi\u00e8mement, l’MS o\u00f9 les produits sont identifi\u00e9s et dans certains cas quantifi\u00e9s.<\/p>\n

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Fig. 1. Une pompe seringue et un potentiostat avec un r\u00e9acteur EC \u00e0 l’int\u00e9rieur.<\/em><\/p>\n

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R\u00e9acteur \u00e9lectrochimique<\/h2>\n

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Dans un r\u00e9acteur \u00e9lectrochimique \u00e0 flux continu, une solution de substrat est introduite au moyen d’une pompe \u00e0 seringue. Le r\u00e9acteur est en fait une cellule \u00e9lectrochimique, un petit potentiel est appliqu\u00e9 sur les \u00e9lectrodes pour entra\u00eener la r\u00e9action. La sortie de la cellule est coupl\u00e9e \u00e0 un MS pour l’analyse des produits.<\/p>\n

Plusieurs param\u00e8tres sont importants pour la r\u00e9ussite d’une exp\u00e9rience EC\/MS. Le premier est le potentiel appliqu\u00e9<\/strong>, il fournit l’\u00e9nergie n\u00e9cessaire pour qu’une r\u00e9action se produise. Un potentiel positif pour les r\u00e9actions d’oxydation et n\u00e9gatif pour les r\u00e9actions de r\u00e9duction. Une fois que les conditions sont \u00e9tablies et que la r\u00e9action se produit, augmenter encore le potentiel n’acc\u00e9l\u00e9rera pas la r\u00e9action et ne g\u00e9n\u00e9rera pas plus de produit. La raison en est expliqu\u00e9e dans les lois traditionnelles de l’\u00e9lectrochimie. Le facteur limitant n’est pas le potentiel, c’est la diffusion du substrat de la solution \u00e0 l’\u00e9lectrode.<\/p>\n

Le deuxi\u00e8me param\u00e8tre important est la composition du solvant<\/strong>. La valeur du pH et le pourcentage en solvant organique peuvent avoir une grande influence. Pour la r\u00e9duction des prot\u00e9ines par exemple, l’acide formique est utilis\u00e9 pour fixer le pH. Mais aussi parce qu’il engendre un interm\u00e9diaire r\u00e9actif qui catalyse la r\u00e9action, d\u00e9crite plus en d\u00e9tail par B. Stocks et J. Melanson (2019<\/span>)<\/a>.<\/p>\n

De plus, le d\u00e9bit<\/strong> est important, typiquement \u00e0 un d\u00e9bit plus faible, un pourcentage relativement plus important du substrat sera converti en produit. La taille des \u00e9lectrodes<\/strong> et l’espacement entre les \u00e9lectrodes<\/strong> sont d’autres facteurs qui jouent un r\u00f4le.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb5%|5%|||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_image src=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2020\/12\/pump-EC-MS.jpg\u00a0\u00bb title_text=\u00a0\u00bbpompe-EC-MS\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Fig. 2. Configuration la plus basique pour EC\/MS. Une pompe \u00e0 seringue fournit le substrat. Dans le r\u00e9acteur EC, des produits d’oxydation ou de r\u00e9duction sont form\u00e9s et analys\u00e9s dans un MS.<\/em><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb admin_label=\u00a0\u00bbDividerline\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb|0px|||false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%|||false|false\u00a0\u00bb locked=\u00a0\u00bboff\u00a0\u00bb collapsed=\u00a0\u00bbon\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_divider color=\u00a0\u00bb#dee1e6″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00a0\u00bb2_3,1_3″ custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb27px|0|27px|0px|false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb|0px||0px||true\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%||0px|false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb2_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb|||\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbSubtitle\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb16px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bb|600||on|||||\u00a0\u00bb header_2_text_color=\u00a0\u00bb#df0834″ header_2_font_size=\u00a0\u00bb20px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bb|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb||0px||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Spectrom\u00e9trie de masse<\/h2>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbParagraph\u00a0\u00bb _builder_version=\u00a0\u00bb4.19.1″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb18px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bbRaleway|700|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb50px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb text_orientation=\u00a0\u00bbjustified\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb0px||||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb35px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb text_orientation_tablet=\u00a0\u00bb\u00a0\u00bb text_orientation_phone=\u00a0\u00bbleft\u00a0\u00bb text_orientation_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Les produits de la r\u00e9action sont analys\u00e9s dans le spectrom\u00e8tre de masse. Ce couplage en ligne pr\u00e9sente plusieurs avantages. Tout d’abord, il est tr\u00e8s rapide<\/strong>. En g\u00e9n\u00e9ral, les produits de la r\u00e9action sont analys\u00e9s par MS en moins de 15 secondes. Ensuite, le solvant est beaucoup plus propre<\/strong> qu’avec les autres m\u00e9thodes. Les m\u00e9thodes traditionnelles n\u00e9cessitent souvent l’ajout de produits chimiques ou d’enzymes, et une \u00e9tape de nettoyage est n\u00e9cessaire avant l’analyse dans un MS. Troisi\u00e8mement, avec une configuration en ligne, il est plus facile d’automatiser<\/strong> un flux de travail, ou d’utiliser un flux d’appoint et d’ajouter des substrats<\/strong> ou d’ajuster le pH du solvant et d’am\u00e9liorer la sensibilit\u00e9 du MS.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb5%|5%|||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_image src=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2020\/12\/pump-EC-MS2.jpg\u00a0\u00bb alt=\u00a0\u00bbConjugaison EC MS\u00a0\u00bb title_text=\u00a0\u00bbpompe-EC-MS2″ admin_label=\u00a0\u00bbImage\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Fig. 3. Configuration EC\/MS en ligne pour \u00e9tudier la conjugaison. Un flux d’appoint avec du substrat est ajout\u00e9 apr\u00e8s le r\u00e9acteur.
<\/em><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb admin_label=\u00a0\u00bbDividerline\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb|0px|||false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%|||false|false\u00a0\u00bb locked=\u00a0\u00bboff\u00a0\u00bb collapsed=\u00a0\u00bbon\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_divider color=\u00a0\u00bb#dee1e6″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00a0\u00bb2_3,1_3″ custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb27px|0|27px|0px|false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb|0px||0px||true\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%||0px|false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb2_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb|||\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbSubtitle\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb16px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bb|600||on|||||\u00a0\u00bb header_2_text_color=\u00a0\u00bb#df0834″ header_2_font_size=\u00a0\u00bb20px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bb|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb||0px||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

\u00c9tudes sur les m\u00e9tabolites pharmaceutiques<\/h2>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbParagraph\u00a0\u00bb _builder_version=\u00a0\u00bb4.19.1″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb18px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bbRaleway|700|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb50px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb text_orientation=\u00a0\u00bbjustified\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb0px||||false|false\u00a0\u00bb hover_enabled=\u00a0\u00bb0″ header_2_font_size_tablet=\u00a0\u00bb35px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb text_orientation_tablet=\u00a0\u00bb\u00a0\u00bb text_orientation_phone=\u00a0\u00bbleft\u00a0\u00bb text_orientation_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb sticky_enabled=\u00a0\u00bb0″]L’un des domaines d’application de l’EC\/MS est l’\u00e9tude des m\u00e9tabolites oxydatifs des produits pharmaceutiques. L’EC\/MS offre des avantages significatifs qui rendent la m\u00e9thode EC compl\u00e9mentaire aux m\u00e9thodes existantes. Les m\u00e9tabolites \u00e0 vie courte et hautement r\u00e9actifs ne peuvent \u00eatre mesur\u00e9s qu’en EC\/MS, et les interactions avec les substrats secondaires et les conjugu\u00e9s sont plus faciles \u00e0 \u00e9tudier (phase II). D’autre part, il a \u00e9galement \u00e9t\u00e9 d\u00e9montr\u00e9 que certaines r\u00e9actions m\u00e9taboliques in vivo<\/em> sont difficiles \u00e0 simuler in vitro<\/em> par les r\u00e9acteurs EC.<\/p>\n

Sur la droite montre un exemple d’EC\/MS de l’amodiaquine (AQ). La trace m\/z de l’AQ dispara\u00eet et les traces des m\u00e9tabolites apparaissent lorsque le r\u00e9acteur est mis en marche. En 5 minutes, tous les m\u00e9tabolites connus de l’AQ sont trouv\u00e9s et identifi\u00e9s. Vous trouverez plus de d\u00e9tails dans notre note d’application<\/a>.<\/p>\n

Cette technique permet la synth\u00e8se \u00e9lectrochimique rapide de m\u00e9tabolites de m\u00e9dicaments en vue de leur identification et de leur quantification par spectrom\u00e9trie de masse (SM). La technique peut \u00eatre utilis\u00e9e dans une configuration \u00e0 flux continu, ce qui permet de la mettre en \u0153uvre dans un flux de travail automatis\u00e9 ou comme syst\u00e8me de criblage \u00e0 haut d\u00e9bit.[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb5%|5%|||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_image src=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2020\/12\/oxidation_amodiaquine.jpg\u00a0\u00bb title_text=\u00a0\u00bboxydation_amodiaquine\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Fig. 3. L’amodiaquine, m\u00e9dicament anti-malaria (trace noire) et ses produits d’oxydation. Les traces m\/z sont surveill\u00e9es pendant que le potentiel du r\u00e9acteur est incr\u00e9ment\u00e9 de 0 \u00e0 2 volts.
<\/em><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb admin_label=\u00a0\u00bbDividerline\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb|0px|||false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%|||false|false\u00a0\u00bb locked=\u00a0\u00bboff\u00a0\u00bb collapsed=\u00a0\u00bbon\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_divider color=\u00a0\u00bb#dee1e6″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00a0\u00bb2_3,1_3″ custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb27px|0|27px|0px|false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb|0px||0px||true\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%||0px|false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb2_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb|||\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbSubtitle\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb16px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bb|600||on|||||\u00a0\u00bb header_2_text_color=\u00a0\u00bb#df0834″ header_2_font_size=\u00a0\u00bb20px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bb|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb||0px||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

\u00c9lucidation de la structure des prot\u00e9ines<\/h2>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbParagraph\u00a0\u00bb _builder_version=\u00a0\u00bb4.19.1″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb18px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bbRaleway|700|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb50px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb text_orientation=\u00a0\u00bbjustified\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb0px||||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb35px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb text_orientation_tablet=\u00a0\u00bb\u00a0\u00bb text_orientation_phone=\u00a0\u00bbleft\u00a0\u00bb text_orientation_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Un autre domaine d’application de l’EC\/MS est celui de la recherche biopharmaceutique, qui conna\u00eet une croissance rapide. Les produits biopharmaceutiques sont des m\u00e9dicaments qui sont produits dans des syst\u00e8mes vivants gr\u00e2ce \u00e0 la biotechnologie. Il s’agit de prot\u00e9ines complexes de poids mol\u00e9culaire \u00e9lev\u00e9, telles que les prot\u00e9ines de fusion, les prot\u00e9ines de bioaffinit\u00e9, les anticorps (mAbs) et les conjugu\u00e9s anticorps-m\u00e9dicaments (ADC).<\/p>\n

Avec le nombre croissant de produits biopharmaceutiques apparaissant sur le march\u00e9, il existe une demande croissante de strat\u00e9gies efficaces et automatis\u00e9es pour l’\u00e9lucidation structural. Cela peut \u00eatre un v\u00e9ritable d\u00e9fi. Ce n’est pas seulement la structure primaire, la cartographie des acides amin\u00e9s qui est importante. Elle concerne \u00e9galement la structure d’ordre sup\u00e9rieur (HOS) d\u00e9finie par les ponts disulfures internes et les modifications post-traductionnelles telles que les phosphorylations, les ac\u00e9tylations, les glycosylations, etc. La structure est ce qui d\u00e9finit l’activit\u00e9 biologique (revue : D. Vir\u00e1g et al, 2020<\/a>).<\/p>\n

Les liaisons disulfures dans les prot\u00e9ines assurent la stabilit\u00e9 et le reploiement, et sont donc d’une importance capitale pour la fonctionnalit\u00e9 biologique. Dans les anticorps monoclonaux, par exemple, les liaisons disulfure maintiennent ensemble la chaine lourde et la chaine l\u00e9g\u00e8re. Il est donc important de v\u00e9rifier la localisation et la connectivit\u00e9 des disulfures dans les prot\u00e9ines th\u00e9rapeutiques. Cela se fait g\u00e9n\u00e9ralement en r\u00e9duisant les liaisons disulfure et en analysant les changements dans les spectres MS (revue : J. Lakudb et al, 2018<\/a>).<\/p>\n

La r\u00e9duction \u00e9lectrochimique peut automatiser cette t\u00e2che en utilisant un r\u00e9acteur EC coupl\u00e9 en ligne avec un MS. Il s’agit d’une petite mais importante \u00e9tape dans le d\u00e9fi de l’\u00e9lucidation de la structure des produits biopharmaceutiques(C. Cramer at al, 2015<\/a>).<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb5%|5%|||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_image src=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2020\/12\/antibodies3.jpg\u00a0\u00bb title_text=\u00a0\u00bbanticorps3″ _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Fig. 5. Les liaisons disulfures (en jaune) jouent un r\u00f4le important dans la structure d’un anticorps.
<\/em><\/p>\n

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<\/em><\/p>\n

[\/et_pb_text][et_pb_image src=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2020\/12\/disulfide_bond_reduction.png\u00a0\u00bb title_text=\u00a0\u00bbR\u00e9duction de la liaison disulfure\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text _builder_version=\u00a0\u00bb4.16″ _module_preset=\u00a0\u00bbdefault\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Fig. 6. R\u00e9duction (rupture) d’une liaison disulfure dans un r\u00e9acteur EC \u00e0 -1 V.
<\/em><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb admin_label=\u00a0\u00bbDividerline\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb|0px|||false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%|||false|false\u00a0\u00bb locked=\u00a0\u00bboff\u00a0\u00bb collapsed=\u00a0\u00bbon\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_divider color=\u00a0\u00bb#dee1e6″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00a0\u00bb2_3,1_3″ custom_padding_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb27px|0|27px|0px|false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb|0px||0px||true\u00a0\u00bb custom_padding_phone=\u00a0\u00bb|10%||0px|false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb2_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb|||\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbSubtitle\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb16px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bb|600||on|||||\u00a0\u00bb header_2_text_color=\u00a0\u00bb#df0834″ header_2_font_size=\u00a0\u00bb20px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bb|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb||0px||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Applications<\/h2>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbParagraph\u00a0\u00bb _builder_version=\u00a0\u00bb4.19.1″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb18px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bbRaleway|700|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb50px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb text_orientation=\u00a0\u00bbjustified\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb0px||||false|false\u00a0\u00bb hover_enabled=\u00a0\u00bb0″ header_2_font_size_tablet=\u00a0\u00bb35px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb text_orientation_tablet=\u00a0\u00bb\u00a0\u00bb text_orientation_phone=\u00a0\u00bbleft\u00a0\u00bb text_orientation_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb sticky_enabled=\u00a0\u00bb0″]Pour plus de d\u00e9tails, consultez \u00e9galement nos notes d’application<\/a> sur l’EC\/MS et une liste de publications<\/a> utilisant des instruments Antec.<\/p>\n

Voir \u00e9galement un enregistrement vid\u00e9o de la pr\u00e9sentation de l’ASMS par le professeur Uwe Karst<\/a> (2010) et une animation informatique<\/a> comparant la m\u00e9thode traditionnelle et la m\u00e9thode EC\/MS pour les \u00e9tudes de m\u00e9tabolites.[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb5%|5%|||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00a0\u00bb1″ admin_label=\u00a0\u00bbDownload Table\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ background_enable_color=\u00a0\u00bboff\u00a0\u00bb use_background_color_gradient=\u00a0\u00bbon\u00a0\u00bb background_color_gradient_stops=\u00a0\u00bb#f6f9fb 50%|#ffffff 100%\u00a0\u00bb background_color_gradient_start=\u00a0\u00bb#f6f9fb\u00a0\u00bb background_color_gradient_start_position=\u00a0\u00bb50%\u00a0\u00bb background_color_gradient_end=\u00a0\u00bb#ffffff\u00a0\u00bb max_width_tablet=\u00a0\u00bb\u00a0\u00bb max_width_phone=\u00a0\u00bb100%\u00a0\u00bb max_width_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb40px||40px||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_row _builder_version=\u00a0\u00bb4.16″ background_color=\u00a0\u00bbrgba(0,0,0,0)\u00a0\u00bb width_tablet=\u00a0\u00bb\u00a0\u00bb width_phone=\u00a0\u00bb\u00a0\u00bb width_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb max_width=\u00a0\u00bb80%\u00a0\u00bb max_width_tablet=\u00a0\u00bb\u00a0\u00bb max_width_phone=\u00a0\u00bb\u00a0\u00bb max_width_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Un r\u00e9acteur \u00e9lectrochimique \u00e0 flux continu avec d\u00e9tection MS est utilis\u00e9 pour l’analyse pharmaceutique. \u00c9tude du m\u00e9tabolisme oxydatif des m\u00e9dicaments et \u00e9lucidation de la structure des produits biopharmaceutiques.<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":16717,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-16827","page","type-page","status-publish","hentry"],"yoast_head":"\n\u00c9lectrochimie avec d\u00e9tection MS - Antec Scientific<\/title>\n<meta name=\"description\" content=\"Un r\u00e9acteur \u00e9lectrochimique \u00e0 flux continu avec d\u00e9tection MS est utilis\u00e9 pour l'analyse pharmaceutique. \u00c9tude du m\u00e9tabolisme oxydatif des m\u00e9dicaments et \u00e9lucidation de la structure des produits biopharmaceutiques.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/antecscientific.com\/fr\/produits\/techniques\/electrochimie-avec-detection-ms\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u00c9lectrochimie avec d\u00e9tection MS - 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