{"id":16727,"date":"2020-07-23T10:19:06","date_gmt":"2020-07-23T10:19:06","guid":{"rendered":"https:\/\/antecscientific.com\/techniques\/detection-electrochimique-pour-hplc\/"},"modified":"2023-05-08T15:47:04","modified_gmt":"2023-05-08T15:47:04","slug":"detection-electrochimique-pour-hplc","status":"publish","type":"page","link":"https:\/\/antecscientific.com\/fr\/produits\/techniques\/detection-electrochimique-pour-hplc\/","title":{"rendered":"D\u00e9tection \u00e9lectrochimique pour HPLC"},"content":{"rendered":"

[et_pb_section fb_built=\u00a0\u00bb1″ admin_label=\u00a0\u00bbHeader\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ background_image=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2020\/10\/Antec_technique_1_2.jpg\u00a0\u00bb parallax=\u00a0\u00bbon\u00a0\u00bb custom_padding=\u00a0\u00bb2vw|0px|200px|0px|false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_row _builder_version=\u00a0\u00bb4.16″ collapsed=\u00a0\u00bbon\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb|||\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbBreadcrumbs (don’t touch)\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb|700|||||||\u00a0\u00bb text_text_color=\u00a0\u00bb#ffffff\u00a0\u00bb text_font_size=\u00a0\u00bb15px\u00a0\u00bb link_font=\u00a0\u00bb|700|||||||\u00a0\u00bb link_text_color=\u00a0\u00bb#ffffff\u00a0\u00bb link_font_size=\u00a0\u00bb15px\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbBig page title\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb header_font=\u00a0\u00bb|700|||||||\u00a0\u00bb header_text_color=\u00a0\u00bb#ffffff\u00a0\u00bb header_font_size=\u00a0\u00bb80px\u00a0\u00bb header_line_height=\u00a0\u00bb1.2em\u00a0\u00bb header_5_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_5_text_color=\u00a0\u00bb#000000″ header_5_font_size=\u00a0\u00bb20px\u00a0\u00bb header_5_letter_spacing=\u00a0\u00bb0.5em\u00a0\u00bb header_5_line_height=\u00a0\u00bb1.8em\u00a0\u00bb animation_style=\u00a0\u00bbzoom\u00a0\u00bb animation_intensity_zoom=\u00a0\u00bb5%\u00a0\u00bb header_font_size_tablet=\u00a0\u00bb48px\u00a0\u00bb header_font_size_phone=\u00a0\u00bb32px\u00a0\u00bb header_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb header_5_font_size_tablet=\u00a0\u00bb14px\u00a0\u00bb header_5_font_size_phone=\u00a0\u00bb12px\u00a0\u00bb header_5_font_size_last_edited=\u00a0\u00bbon|desktop\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

D\u00e9tection \u00e9lectrochimique pour HPLC<\/h1>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=\u00a0\u00bb1″ admin_label=\u00a0\u00bbContent\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ background_color=\u00a0\u00bb#ffffff\u00a0\u00bb max_width=\u00a0\u00bb90%\u00a0\u00bb max_width_tablet=\u00a0\u00bb\u00a0\u00bb max_width_phone=\u00a0\u00bb100%\u00a0\u00bb max_width_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb custom_margin=\u00a0\u00bb-160px||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb||0px||false|false\u00a0\u00bb border_color_all=\u00a0\u00bb#ffffff\u00a0\u00bb border_width_left=\u00a0\u00bb10vw\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_row column_structure=\u00a0\u00bb2_3,1_3″ custom_padding_last_edited=\u00a0\u00bboff|desktop\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb27px|0|27px|0px|false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb|0px||0px||true\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb2_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb|||\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbSubtitle\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb16px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bb|600||on|||||\u00a0\u00bb header_2_text_color=\u00a0\u00bb#df0834″ header_2_font_size=\u00a0\u00bb20px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bb|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb||0px||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Qu’est-ce que la d\u00e9tection \u00e9lectrochimique ?<\/h2>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbParagraph\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb18px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bbRaleway|700|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb50px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb text_orientation=\u00a0\u00bbjustified\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb0px||||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb35px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

La d\u00e9tection \u00e9lectrochimique (DCE) pour HPLC ou uHPLC est une technique de d\u00e9tection s\u00e9lective et extr\u00eamement sensible qui est appliqu\u00e9e dans un certain nombre d’analyses telles que les neurotransmetteurs, les cat\u00e9cholamines, les aminoglycosides, les glucides, les thiols et les ph\u00e9nols. Pour la d\u00e9tection, on utilise des cellules \u00e0 \u00e9coulement et des \u00e9lectrodes qui peuvent \u00eatre miniaturis\u00e9es, ce qui permet de l’adapter \u00e0 la HPLC standard ou nanom\u00e9trique. En combinaison avec l’\u00e9lectronique appropri\u00e9e, l’ECD a une \u00e9norme gamme dynamique lin\u00e9aire de plus de 7 ordres de grandeur. Cela signifie que les concentrations peuvent \u00eatre mesur\u00e9es lin\u00e9airement de 10 pmole\/L \u00e0 100 \u00b5mol\/L avec le m\u00eame instrument.<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=\u00a0\u00bb1_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb5%|5%|||false|false\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_image src=\u00a0\u00bbhttps:\/\/antecscientific.com\/wp-content\/uploads\/2020\/08\/ALX_Elite2.jpg\u00a0\u00bb title_text=\u00a0\u00bbALX_Elite2″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_image][et_pb_text admin_label=\u00a0\u00bbimage explanation\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb13px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bbRaleway|700|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb50px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb35px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

HPLC avec un d\u00e9tecteur \u00e9lectrochimique.<\/em><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row admin_label=\u00a0\u00bbDividerline\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb collapsed=\u00a0\u00bbon\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb4_4″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_divider color=\u00a0\u00bb#dee1e6″ _builder_version=\u00a0\u00bb4.16″ global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=\u00a0\u00bb2_3,1_3″ custom_padding_last_edited=\u00a0\u00bboff|desktop\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ width=\u00a0\u00bbauto\u00a0\u00bb max_width=\u00a0\u00bbnone\u00a0\u00bb custom_padding=\u00a0\u00bb27px|0|27px|0px|false|false\u00a0\u00bb custom_padding_tablet=\u00a0\u00bb|0px||0px||true\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb][et_pb_column type=\u00a0\u00bb2_3″ _builder_version=\u00a0\u00bb4.16″ custom_padding=\u00a0\u00bb|||\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb custom_padding__hover=\u00a0\u00bb|||\u00a0\u00bb][et_pb_text admin_label=\u00a0\u00bbSubtitle\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb16px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bb|600||on|||||\u00a0\u00bb header_2_text_color=\u00a0\u00bb#df0834″ header_2_font_size=\u00a0\u00bb20px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bb|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb||0px||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb||0px||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Comment fonctionne la d\u00e9tection \u00e9lectrochimique ?<\/h2>\n

[\/et_pb_text][et_pb_text admin_label=\u00a0\u00bbParagraph\u00a0\u00bb _builder_version=\u00a0\u00bb4.16″ text_font=\u00a0\u00bb||||||||\u00a0\u00bb text_font_size=\u00a0\u00bb18px\u00a0\u00bb text_line_height=\u00a0\u00bb2em\u00a0\u00bb header_font=\u00a0\u00bb||||||||\u00a0\u00bb header_2_font=\u00a0\u00bbRaleway|700|||||||\u00a0\u00bb header_2_font_size=\u00a0\u00bb50px\u00a0\u00bb header_2_line_height=\u00a0\u00bb1.4em\u00a0\u00bb header_6_font=\u00a0\u00bbRaleway|700||on|||||\u00a0\u00bb header_6_font_size=\u00a0\u00bb16px\u00a0\u00bb header_6_letter_spacing=\u00a0\u00bb5px\u00a0\u00bb header_6_line_height=\u00a0\u00bb1.8em\u00a0\u00bb text_orientation=\u00a0\u00bbjustified\u00a0\u00bb max_width=\u00a0\u00bb700px\u00a0\u00bb module_alignment=\u00a0\u00bbcenter\u00a0\u00bb custom_margin=\u00a0\u00bb0px||||false|false\u00a0\u00bb custom_padding=\u00a0\u00bb0px||||false|false\u00a0\u00bb header_2_font_size_tablet=\u00a0\u00bb35px\u00a0\u00bb header_2_font_size_phone=\u00a0\u00bb25px\u00a0\u00bb header_2_font_size_last_edited=\u00a0\u00bbon|phone\u00a0\u00bb global_colors_info=\u00a0\u00bb{}\u00a0\u00bb]<\/p>\n

Dans la d\u00e9tection \u00e9lectrochimique amp\u00e9rom\u00e9trique, on mesure le courant \u00e9lectrique r\u00e9sultant des r\u00e9actions d’oxydation ou de r\u00e9duction (voir vid\u00e9o). Un \u00e9chantillon est introduit dans la HPLC et s\u00e9par\u00e9 sur la colonne chromatographique. La colonne est connect\u00e9e \u00e0 une cellule ECD, qui est un capteur \u00e9lectrochimique o\u00f9 une r\u00e9action a lieu au niveau d’une \u00e9lectrode. Les substances \u00e9lectrochimiquement actives qui \u00e9luent de la colonne subissent une r\u00e9action \u00e9lectrochimique, les \u00e9lectrons sont transf\u00e9r\u00e9s et il en r\u00e9sulte un courant \u00e9lectrique. Les \u00e9lectrodes sont connect\u00e9es \u00e0 un circuit \u00e9lectronique dot\u00e9 d’un puissant amplificateur \u00e0 faible bruit qui convertit un courant de l’ordre du pico- ou du nanoamp\u00e8re en un signal d\u00e9tectable pour l’acquisition de donn\u00e9es.<\/p>\n

Lire la suite :<\/p>\n