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La intolerancia a la lactosa es una enfermedad muy extendida, que impide a muchas personas consumir productos l\u00e1cteos. El mercado mundial de productos l\u00e1cteos \u00absin lactosa\u00bb est\u00e1 creciendo r\u00e1pidamente, y los criterios para etiquetar productos \u00absin lactosa\u00bb son cada vez m\u00e1s estrictos. Para comprobar el contenido de lactosa en estos productos, se necesitan m\u00e9todos anal\u00edticos r\u00e1pidos, sensibles y selectivos.<\/p>\n
En esta nota de aplicaci\u00f3n se presenta un m\u00e9todo para el an\u00e1lisis r\u00e1pido y sensible de la lactosa y sus is\u00f3meros, como la lalolactosa, la epilactosa y la lactulosa. El m\u00e9todo se basa en la cromatograf\u00eda de intercambio ani\u00f3nico de alto rendimiento con detecci\u00f3n amperom\u00e9trica pulsada (HPAE-PAD). Se analizaron diversos productos l\u00e1cteos para demostrar la versatilidad del m\u00e9todo.<\/p>\n
Adem\u00e1s de la cuantificaci\u00f3n de bajas concentraciones de lactosa y sus is\u00f3meros, tambi\u00e9n pueden analizarse con el m\u00e9todo presentado los principales az\u00facares de los productos l\u00e1cteos (galactosa, glucosa, sacarosa y fructosa).<\/p>\n
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