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El Analizador de Neurotransmisores ALEXYS puede utilizarse para la detecci\u00f3n r\u00e1pida y sensible de los niveles basales de acetilcolina (ACh) en muestras de microdi\u00e1lisis. El an\u00e1lisis de ACh por HPLC-ECD se basa en una separaci\u00f3n por apareamiento i\u00f3nico, seguida de una conversi\u00f3n enzim\u00e1tica post-columna en per\u00f3xido de hidr\u00f3geno con acetilcolinesterasa (AChE) y colina oxidasa (ChO). Ambas enzimas se unen covalentemente a una fase estacionaria en un reactor enzim\u00e1tico inmovilizado (IMER). Tras la conversi\u00f3n, el per\u00f3xido de hidr\u00f3geno se detecta utilizando el detector electroqu\u00edmico DECADE Elite con Sencell en un electrodo de Pt. El m\u00e9todo est\u00e1 optimizado para el an\u00e1lisis de peque\u00f1os vol\u00famenes de muestra con un bajo l\u00edmite de detecci\u00f3n nanomolar.<\/p>\n
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