{"id":14892,"date":"2020-07-23T10:19:06","date_gmt":"2020-07-23T10:19:06","guid":{"rendered":"https:\/\/antecscientific.com\/tecnicas\/deteccion-electroquimica-para-hplc\/"},"modified":"2023-01-09T12:21:45","modified_gmt":"2023-01-09T12:21:45","slug":"deteccion-electroquimica-para-hplc","status":"publish","type":"page","link":"https:\/\/antecscientific.com\/es\/productos\/tecnicas\/deteccion-electroquimica-para-hplc\/","title":{"rendered":"Detecci\u00f3n electroqu\u00edmica para HPLC"},"content":{"rendered":"

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Detecci\u00f3n electroqu\u00edmica para HPLC<\/h1>\n

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\u00bfQu\u00e9 es la detecci\u00f3n electroqu\u00edmica?<\/h2>\n

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La detecci\u00f3n electroqu\u00edmica (ECD) para HPLC o uHPLC es una t\u00e9cnica de detecci\u00f3n selectiva y extremadamente sensible que se aplica en numerosos an\u00e1lisis, como neurotransmisores, catecolaminas, aminogluc\u00f3sidos, hidratos de carbono, tioles y fenoles. Para la detecci\u00f3n, se utilizan celdas de flujo y electrodos que pueden miniaturizarse, lo que los hace aptos para la HPLC est\u00e1ndar y la nano-HPLC. En combinaci\u00f3n con la electr\u00f3nica adecuada, el ECD tiene un enorme rango din\u00e1mico lineal de m\u00e1s de 7 \u00f3rdenes de magnitud. Esto significa que las concentraciones pueden medirse linealmente desde 10 pmole\/L hasta 100 \u00b5mol\/L con el mismo instrumento.<\/p>\n

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HPLC con detector electroqu\u00edmico.<\/em><\/p>\n

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\u00bfC\u00f3mo funciona la detecci\u00f3n electroqu\u00edmica?<\/h2>\n

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En la detecci\u00f3n electroqu\u00edmica amperom\u00e9trica se mide la corriente el\u00e9ctrica resultante de las reacciones de oxidaci\u00f3n o reducci\u00f3n (ver v\u00eddeo). Se introduce una muestra en la HPLC y se separa en la columna cromatogr\u00e1fica. La columna est\u00e1 conectada a una c\u00e9lula ECD, que es un sensor electroqu\u00edmico en el que se produce una reacci\u00f3n en un electrodo. Las sustancias electroqu\u00edmicamente activas que eluyen de la columna sufren una reacci\u00f3n electroqu\u00edmica, los electrones se transfieren dando lugar a una corriente el\u00e9ctrica. Los electrodos est\u00e1n conectados a un circuito electr\u00f3nico con un potente amplificador de bajo ruido que convierte una corriente de pico o nanoamperios en una se\u00f1al detectable para la adquisici\u00f3n de datos.<\/p>\n

M\u00e1s informaci\u00f3n:<\/p>\n